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Fig. 1 (a) shows a Golgi apparatus bearing typical glandular architecture on the surface of a representative growth cone. A single PilT molecule is shown on the surface. Inset shows the zoomed-in view of the PilT interacting with 19:0/18:1 PS. (Figure 1 (b) Green and red shows the number of PilT and PilQ molecules in a cell respectively. [Figure 1 (c)](#f1){ref-type="fig"} shows paths of individual PilT molecules on the surface of growth cone cell surface. Each diamorphic field represents one PilT insertion or retraction event. PilT switching duration decreases with increasing diameter of the filament. The green divergence on the surface represents the pilus retraction direction. Retraction of a pilus is due to the pulling of the tip by the retraction apparatus (RA). The size of RA is dictated by the [precedent]{.ul} recurrence time of TFP retraction distance before stalling. The strategies for controlling TFP mediated transport also involve the spatio-temporal distribution of pilus [retraction]{.ul} on the growth cone surface. In case of a pilus that is too long or too weak, it is easily stripped off by the cellular receptor. Thus there are two interactions, one that occurs before the [tension]{.ul} is imposed by the RA. The key to [recruiting]{.ul} pilins is the pilus retraction process itself. The pilus is flexible enough to be cycled rapidly and thus may be recycled multiple times. This allows the pilus to [recruit]{.ul} more pilins from the cell periplasm. Figure 3 shows snapshots of three cases of pilus retraction. The figure 3 (b) and (c) both depict a case of TFP retraction before stalling. The figure 3(a) shows a case of TFP retraction after stalling. The figures show the PilT and needles (RA) interactions with 19:0/18:1PS. The figure (a) shows a case of TFP pulling on the tip of RA which reduces its size. [figure]{.ul} (b) depicts a case of TFP pulling a needle from the cell which reduces its size. [Figure]{.
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