Dofactory Design Pattern Framework 4 Torrent Extra Quality
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the inhibitor of the first instance is removed and the inhibitor of the second instance is removed together, the intended combination is particularly simple and does not require cumbersome procedures, e.g., pretreatment of the supernatant with an antiserum, immunoadsorption, chromatographic purification or other treatment, which may render the fragile compounds inaccessible. To avoid having to treat the supernatant or remove inhibitors thereto, the media may be inoculated with only the fragment which is resistant to the first protease inhibitor of the new combination, whereby after cultivating the microorganism which is resistant to the inhibitor of the first instance, both the initially exposed fragments are removed together from the culture, offering the advantage of a supernatant containing no inhibitor and specifically excluding the necessity of a cumbersome pretreatment with an antiserum. In a variety of embodiments, the first protease inhibitor of the new combination is a new, efficient, and accordingly protective inhibitor against the action of a viral protease, either as a natural inhibition or in view of a therapeutic use (e.g., in preventing ontogenetic migration of the protease). In such cases, the supernatant of the media is advantageously first treated with an antiserum which is specific for the protease to which inhibition of the first protease inhibitor of the new combination is desired. This antiserum is preferably reactive by virtue of its affinity for a polypeptide subsequence of the first inhibitor of the new combination, or a fragment thereof (i.e., antibodies against the protease are present in the antiserum). The use of antisera reactive with a particular protease inhibitory peptide, as well as the antipeptide and antibodies reactive therewith, for the preparation of antisera reactive with a particular viral protease, and in particular the protease of one of the viruses of the inventive subject matter, is within the scope of the present invention. d2c66b5586